| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.214779 |
| Chromosome: | chromosome_4 |
| Location: | 340140 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g217908 | FAP79 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGGCATGCAGGGCCGAGGGTCGGGACTCC |
| Internal bar code: | CCTTAGAGGTCAGCTCTTGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1257 |
| LEAP-Seq percent confirming: | 79.8069 |
| LEAP-Seq n confirming: | 4711 |
| LEAP-Seq n nonconfirming: | 1192 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTTCACTAAGGCGTCCA |
| Suggested primer 2: | GGACAGGATCCAAGAGGTGA |