| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214785 |
| Chromosome: | chromosome 5 |
| Location: | 2317497 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234801 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTCACCTCGCGACGACTCTGCAATGTG |
| Internal bar code: | CAATTTGCGAAGTGTGAGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 99.7932 |
| LEAP-Seq n confirming: | 4826 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCCTCCATTTGATGTTT |
| Suggested primer 2: | TTACAAGGAATACCAGCGGC |