| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.214798 |
| Chromosome: | chromosome 3 |
| Location: | 7849690 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202851 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATAGGTAATATGCGCAGACTGGAGGCCA |
| Internal bar code: | GTATCCGTGGCGACGTAGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 98.233 |
| LEAP-Seq n confirming: | 10285 |
| LEAP-Seq n nonconfirming: | 185 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGTTGCTTTGACAACTT |
| Suggested primer 2: | GTGTGGATGATGACCTGTCG |