| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214798 |
| Chromosome: | chromosome 3 |
| Location: | 9041904 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210401 | (1 of 1) PTHR14614//PTHR14614:SF15 - HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCAGTGACGTAGACGTGTTTCGGTTGA |
| Internal bar code: | GGTTCTCTATATATAGCGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.8418 |
| LEAP-Seq n confirming: | 1262 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTCGATGCTCTGTGGTA |
| Suggested primer 2: | AGAGCCACCCAAATGTCAAC |