| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214832 |
| Chromosome: | chromosome_3 |
| Location: | 5070740 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g181100 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGCCCCTAGCGTGATGGAACATGGCAAGG |
| Internal bar code: | GGGTAGCGAGTAGCTTCTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1008 |
| LEAP-Seq percent confirming: | 99.7633 |
| LEAP-Seq n confirming: | 5057 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAGCTTCCCCATCTCTT |
| Suggested primer 2: | TTATCTTGCCTCGTGTGCAG |