| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214854 |
| Chromosome: | chromosome 13 |
| Location: | 3548577 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588100 | CYN19C,ROC2,CYN5,CYN19-3 | (1 of 1) K09565 - peptidyl-prolyl isomerase F (cyclophilin D) (PPIF); Cyclophilin 19-3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCTGGCACATGAAGGAGGGGATGACGCG |
| Internal bar code: | GCGTTATGACTGAGATGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1015 |
| LEAP-Seq percent confirming: | 99.7027 |
| LEAP-Seq n confirming: | 12745 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCAATGATACTCAGGCA |
| Suggested primer 2: | CAAGACTGCGGAGAACTTCC |