Insertion junction: LMJ.RY0402.214865_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.214865
Chromosome:chromosome_7
Location:647918
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g317100 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TACAGTTGTGACTTGTCAGGGTGGAGGTTT
Internal bar code:TGGATTATATACGTCGTCCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:204
LEAP-Seq percent confirming:98.5667
LEAP-Seq n confirming:894
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTTCGTTGGTTTTGGTT
Suggested primer 2:CACTTCCGGACACACACATC