| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214897 |
| Chromosome: | chromosome 12 |
| Location: | 7082512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559450 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGAGGGGATGAGCACGAAGTCAAACT |
| Internal bar code: | GACTAGTCCCAGCCACGTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 264 |
| LEAP-Seq percent confirming: | 99.6718 |
| LEAP-Seq n confirming: | 4859 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATAGAACGGGAATGGGCTG |
| Suggested primer 2: | GAGGGTAGCCGTTCATGTGT |