| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214899 |
| Chromosome: | chromosome 16 |
| Location: | 2012500 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656851 | (1 of 2) PF07856 - Mediator of CRAC channel activity (Orai-1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACGGAGCAGCAGCGGTGGGCATGGAGC |
| Internal bar code: | CGGTTTCCGTTCAGCCTCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 99.3675 |
| LEAP-Seq n confirming: | 4399 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTCGGTACCACTCCCAA |
| Suggested primer 2: | GGGCTATGTGATTAAGCCGA |