| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214918 |
| Chromosome: | chromosome 13 |
| Location: | 2648342 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581550 | THG1 | Histidine tRNA 5'-guanylyltransferase; (1 of 1) 2.7.7.79 - tRNA(His) guanylyltransferase / Histidine tRNA guanylyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGCAGGATCGCCGGGCAGGGGAGTATC |
| Internal bar code: | GCCGCGCAATTCGTGCTCATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 99.7314 |
| LEAP-Seq n confirming: | 9284 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGGTGCCTTAAGTCGTT |
| Suggested primer 2: | GGGGTTGCTCAGGTTGTAGA |