| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214955 |
| Chromosome: | chromosome 13 |
| Location: | 2292017 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTGCAGCAGCATGGACCAACGTTGCCTG |
| Internal bar code: | AACGTGTATGGATGTGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 862 |
| LEAP-Seq percent confirming: | 85.1219 |
| LEAP-Seq n confirming: | 349 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTCCTGCATCTTACGCA |
| Suggested primer 2: | TCTATCAACCCGTCCTCGTC |