| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214962 |
| Chromosome: | chromosome 9 |
| Location: | 7246000 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412550 | (1 of 3) IPR000104//IPR005069 - Antifreeze protein, type I // Nucleotide-diphospho-sugar transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAAAATGGCGAAGGCCCCACACGAACGC |
| Internal bar code: | CGGTTTGTCAGACAAACAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 970 |
| LEAP-Seq percent confirming: | 92.3274 |
| LEAP-Seq n confirming: | 25210 |
| LEAP-Seq n nonconfirming: | 2095 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGAAAAGGGTTGCGATT |
| Suggested primer 2: | CCGCTTCATGATTTAACCGT |