| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214968 |
| Chromosome: | chromosome 11 |
| Location: | 1399686 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467721 | (1 of 1) IPR027446 - Variant surface glycoprotein C-terminal domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTGTGAAGATAACAGTACACCATGACC |
| Internal bar code: | GTGCGGCGGCACTTCCTATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 98.8042 |
| LEAP-Seq n confirming: | 661 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAAGGGGACTCACAATG |
| Suggested primer 2: | ACCTACCGACAGCGAGAAGA |