Insertion junction: LMJ.RY0402.214991_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.214991
Chromosome:chromosome 7
Location:2967057
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g333000 CEP4 (1 of 1) 3.4.22.15 - Cathepsin L; Cysteine endopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCAAAACTCCATTTCTGGTAGGGGCTAC
Internal bar code:TGCAAATGTGGCGAGAATCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:736
LEAP-Seq percent confirming:99.3464
LEAP-Seq n confirming:4256
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TATACCAGCAGCAGCACCAG
Suggested primer 2:TGACGTACGTACCCAAACGA