| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215001 |
| Chromosome: | chromosome 6 |
| Location: | 8232289 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305750 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGGCAAGCAGGATCCATACGTGAAATT |
| Internal bar code: | CCAATAGGCCGGGATTTGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 98.9583 |
| LEAP-Seq n confirming: | 855 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGTCGCGAACTTTGGTG |
| Suggested primer 2: | GAAGGAGGAGTGATGGGTGA |