| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215002 |
| Chromosome: | chromosome 2 |
| Location: | 5371826 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107200 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGAGCGCGTTTCGCTGAAGCATGCTGCC |
| Internal bar code: | CAACCCAGTTTGACAATATATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 99.5769 |
| LEAP-Seq n confirming: | 7295 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGGGCTTGTTCATCAGG |
| Suggested primer 2: | TACGATACGATTTCCCTCGC |