Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.215006 |
Chromosome: | chromosome_14 |
Location: | 3707764 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g631900 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGACACAATGTTCCGCAGAGCGGCAAGTC |
Internal bar code: | ATGACTTCAAACGAGCAAGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 996 |
LEAP-Seq percent confirming: | 99.5397 |
LEAP-Seq n confirming: | 3676 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTCAGTGGGGGTCTAAAT |
Suggested primer 2: | CGCTCGTAATTGTCAAGCAA |