Insertion junction: LMJ.RY0402.215015_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.215015
Chromosome:chromosome 1
Location:53087
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g000250 SNR1 (1 of 2) PTHR12677:SF5 - TVP38/TMEM64 FAMILY MEMBRANE PROTEIN YDJX; Predicted SNARE-associated Golgi protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCAACGGTCCCCGCCCTGCCCCTCCCACA
Internal bar code:TTTATTCATTTAACGTTGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:460
LEAP-Seq percent confirming:98.9011
LEAP-Seq n confirming:90
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTATTCGTTCTTTCCGCTGC
Suggested primer 2:GCAATGCGTACGGAACCTAT