| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215015 |
| Chromosome: | chromosome_1 |
| Location: | 53087 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g000250 | SNR1 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCAACGGTCCCCGCCCTGCCCCTCCCACA |
| Internal bar code: | TTTATTCATTTAACGTTGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 98.9011 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATTCGTTCTTTCCGCTGC |
| Suggested primer 2: | GCAATGCGTACGGAACCTAT |