| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215032 |
| Chromosome: | chromosome 5 |
| Location: | 1009790 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246551 | (1 of 1) IPR000104//IPR001810//IPR020683 - Antifreeze protein, type I // F-box domain // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCATGCGGCAATTCAACCCGGCAAGCAA |
| Internal bar code: | ACGTGTTCGAACTCACCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 541 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCACACTGTCACCTCCCC |
| Suggested primer 2: | TCCTGTACCGCTTTTCCAAC |