Insertion junction: LMJ.RY0402.215039_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.215039
Chromosome:chromosome 6
Location:7019440
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g296850 FAP81,DLEC1 (1 of 8) IPR008962 - PapD-like; Flagellar Associated Protein 81 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTATTGGCCCAGGCGTGGCGACAAAACCG
Internal bar code:CAATGTGCAGTGGTACTCTACC

Confirmation - LEAP-Seq

LEAP-Seq distance:63
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCACGTCAAGGTAAAGGG
Suggested primer 2:TGTGAGGCGTTTTGTAGGTG