| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215049 |
| Chromosome: | chromosome_10 |
| Location: | 5448836 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g458600 | FAP245 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATAAGTCTTACTTACCGCGATCGTACTTAC |
| Internal bar code: | CAGATCGCGTCGTTTGATCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 97.902 |
| LEAP-Seq n confirming: | 37098 |
| LEAP-Seq n nonconfirming: | 795 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTCCTGTGGGGACAATA |
| Suggested primer 2: | GAAGCCAACCAGAAGCTGAC |