| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215068 |
| Chromosome: | chromosome 1 |
| Location: | 6778241 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048850 | (1 of 1) PF01342//PF01388 - SAND domain (SAND) // ARID/BRIGHT DNA binding domain (ARID) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCTGGGACGCCAGGGGTGAAGGGCGTGA |
| Internal bar code: | GTAGGATCGCGTGTGCTAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1089 |
| LEAP-Seq percent confirming: | 99.5764 |
| LEAP-Seq n confirming: | 15516 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTACTGTCGTCGCTGTC |
| Suggested primer 2: | CATCGGTAGCAATTGTGTGG |