| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215099 |
| Chromosome: | chromosome 16 |
| Location: | 1730512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654900 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCCCTCCACCACGCGTCCAAAGGTGCC |
| Internal bar code: | GGACTGTCACTCGTGTTCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 857 |
| LEAP-Seq percent confirming: | 99.7046 |
| LEAP-Seq n confirming: | 9452 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCTGCTTCTTCATGTGG |
| Suggested primer 2: | CCACTGACTCACCCTGGTTT |