| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215113 |
| Chromosome: | chromosome 1 |
| Location: | 5077329 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035200 | (1 of 13) 2.3.1.225 - Protein S-acyltransferase / S-protein acyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTGTTGGTGCGAGTGGATACGCTTATG |
| Internal bar code: | CCCAATCATCCTTTTGCTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 407 |
| LEAP-Seq percent confirming: | 98.2658 |
| LEAP-Seq n confirming: | 14676 |
| LEAP-Seq n nonconfirming: | 259 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTGCCTCCCACTCTCAC |
| Suggested primer 2: | TTGCCTTCTCAACAAATCCC |