| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215121 |
| Chromosome: | chromosome 12 |
| Location: | 3785339 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515301 | (1 of 4) PF13515 - Fusaric acid resistance protein-like (FUSC_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCTTGCGCTCCACCTGGCTCGTTTCAC |
| Internal bar code: | GTGTGCTGCGACCGTCTCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 80.4223 |
| LEAP-Seq n confirming: | 4609 |
| LEAP-Seq n nonconfirming: | 1122 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGTCCCCACTGTTGATG |
| Suggested primer 2: | TAGCAGCAGTAGCAGCAGGA |