| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215128 |
| Chromosome: | chromosome 11 |
| Location: | 2764985 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477250 | (1 of 1) PF10187 - N-terminal domain of NEFA-interacting nuclear protein NIP30 (Nefa_Nip30_N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCAACGATTGGGGCACGCGATGCAGGA |
| Internal bar code: | AAGGGAACCAGGTATACAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1091 |
| LEAP-Seq percent confirming: | 97.8006 |
| LEAP-Seq n confirming: | 49535 |
| LEAP-Seq n nonconfirming: | 1114 |
| LEAP-Seq n unique pos: | 228 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTCTGTGGGTTTGTCGT |
| Suggested primer 2: | ATGTAGTTGCTTCCGGTTCG |