| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215136 |
| Chromosome: | chromosome 4 |
| Location: | 3381956 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227500 | SRR18 | Scavenger receptor cysteine rich (SRCR) protein; (1 of 2) IPR001190//IPR006626//IPR011050//IPR017448 - SRCR domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // SRCR-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACGCAAATGCACCGCTGCCCCAAGCCC |
| Internal bar code: | GTTGAGCGGCAGTCTATAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1294 |
| LEAP-Seq percent confirming: | 99.7995 |
| LEAP-Seq n confirming: | 7964 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATATGACGCCCAGCAAAAC |
| Suggested primer 2: | CTACCCATGTGTGCTGGATG |