| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215141 |
| Chromosome: | chromosome 4 |
| Location: | 1511352 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213800 | (1 of 1) PTHR10825//PTHR10825:SF5 - RING FINGER DOMAIN-CONTAINING, POLYCOMB GROUP COMPONENT // E3 UBIQUITIN-PROTEIN LIGASE RING1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGCGGGTGCAATTCATCGTTGTGGATG |
| Internal bar code: | TTTCCCAGGACCTCTTGTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 99.1177 |
| LEAP-Seq n confirming: | 13593 |
| LEAP-Seq n nonconfirming: | 121 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGTCGTGATTTGAAGC |
| Suggested primer 2: | TGATGTTGTTTCGTTGCCAT |