| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215147 |
| Chromosome: | chromosome 7 |
| Location: | 1202753 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321050 | (1 of 1) PTHR11772//PTHR11772:SF3 - ASPARAGINE SYNTHETASE // ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGGCGATGTTCATGGCTACCGCATGG |
| Internal bar code: | CAAGTCGTCCGGAAAGTTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1253 |
| LEAP-Seq percent confirming: | 99.6866 |
| LEAP-Seq n confirming: | 11133 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAAATGCATGGGGAGAGT |
| Suggested primer 2: | GTGTGCTGGCAGGATTACCT |