Insertion junction: LMJ.RY0402.215149_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.215149
Chromosome:chromosome 9
Location:4755892
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g397216 (1 of 3) K01832 - thromboxane-A synthase (TBXAS1, CYP5A) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACCCACCATGGTCGCAAACTTGCTGC
Internal bar code:CGACTGTGTGGGCCGGAGGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:39
LEAP-Seq percent confirming:47.0852
LEAP-Seq n confirming:105
LEAP-Seq n nonconfirming:118
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAGAGCACAACGGAGAGGAG
Suggested primer 2:TGTGTCGAACAAATCGCTTC