| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215150 |
| Chromosome: | chromosome 17 |
| Location: | 3446042 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724650 | (1 of 9) PTHR13789//PTHR13789:SF186 - MONOOXYGENASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGCGGGCCGGCATGCGGCGGGGCGTCG |
| Internal bar code: | GCTGCCCAGCCGGCACAGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1036 |
| LEAP-Seq percent confirming: | 98.4536 |
| LEAP-Seq n confirming: | 65068 |
| LEAP-Seq n nonconfirming: | 1022 |
| LEAP-Seq n unique pos: | 189 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCACCCCACTCTTGAATC |
| Suggested primer 2: | CCATACACCGCTTATGGCTT |