Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.215166 |
Chromosome: | chromosome 16 |
Location: | 6541742 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g672602 | (1 of 30) PTHR24349:SF79 - NITROGEN NETWORK KINASE 1 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCACAGGGGCCATCAAAACGTCCGTCCC |
Internal bar code: | CGATAGGGCTATCTGAACATTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 736 |
LEAP-Seq percent confirming: | 99.7591 |
LEAP-Seq n confirming: | 8282 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAAGAGAAGGTCAAGTGCC |
Suggested primer 2: | TTTTAGCCAGCCAGCAACTT |