| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215179 |
| Chromosome: | chromosome 12 |
| Location: | 4607335 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGTACTCACGGAGCCAATGTGTTAAGC |
| Internal bar code: | TGTTACTTGAATTAGGAACTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 743 |
| LEAP-Seq percent confirming: | 83.5996 |
| LEAP-Seq n confirming: | 1570 |
| LEAP-Seq n nonconfirming: | 308 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCTTGCATGCCGTTGTA |
| Suggested primer 2: | CATGCGGTGTGTCATAAAGG |