| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215186 |
| Chromosome: | chromosome 8 |
| Location: | 4436860 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382200 | (1 of 4) PTHR11584//PTHR11584:SF377 - SERINE/THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACGTACGGGGCTCGGCGGCATGCTTGT |
| Internal bar code: | GTGTAGTTCTCGCCGCTCCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1078 |
| LEAP-Seq percent confirming: | 99.0992 |
| LEAP-Seq n confirming: | 35312 |
| LEAP-Seq n nonconfirming: | 321 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCGTACACCATAAGCCG |
| Suggested primer 2: | TGTCTCGACATACTGCGCTC |