Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.215192 |
Chromosome: | chromosome_10 |
Location: | 4112897 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g449750 | PKS1 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGGATGTGCTCAACGTGCTGGGCATGTAC |
Internal bar code: | CGGTTGATCGCACGATAGGATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 522 |
LEAP-Seq percent confirming: | 91.9525 |
LEAP-Seq n confirming: | 2868 |
LEAP-Seq n nonconfirming: | 251 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTCAGACCCCTGTGAGGAC |
Suggested primer 2: | GGTCAGACTGTTCAGCACCA |