| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215196 |
| Chromosome: | chromosome 4 |
| Location: | 2100512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219300 | (1 of 1) PF02958 - Ecdysteroid kinase (EcKinase); Putative choline kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTTGCGGATGAGGTGAGGCAAGGCACG |
| Internal bar code: | CTAGTGAGACAGTCCCGCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1083 |
| LEAP-Seq percent confirming: | 99.6858 |
| LEAP-Seq n confirming: | 5394 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGGTTGGACGAGTATG |
| Suggested primer 2: | CGGTCACCAGGCGTAAGTAT |