| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215199 |
| Chromosome: | chromosome 6 |
| Location: | 942438 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255450 | MAT3,RB | (1 of 1) K04681 - retinoblastoma-like protein 1 (RBL1); Retinoblastoma protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTTGACAGTGGGGTGTGCGTAGTCATT |
| Internal bar code: | AGCTTAACGGTGTGGGTTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 98.2209 |
| LEAP-Seq n confirming: | 4803 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGAGGAAGAGGAGGTCG |
| Suggested primer 2: | TGTATGTCGAGAGACAGGCG |