| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215199 |
| Chromosome: | chromosome 12 |
| Location: | 5713866 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532750 | EEP8 | (1 of 1) K10772 - AP endonuclease 2 (APEX2); Endonuclease/exonuclease/phosphatase family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAAGGTCCACCCACAGAGGCGCGTGAT |
| Internal bar code: | GGTAGACGAATATGGTTCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 803 |
| LEAP-Seq percent confirming: | 98.0879 |
| LEAP-Seq n confirming: | 35499 |
| LEAP-Seq n nonconfirming: | 692 |
| LEAP-Seq n unique pos: | 134 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTAGCAGCACAAACTG |
| Suggested primer 2: | TGCGTAGGAGTTGGTGTCAG |