Insertion junction: LMJ.RY0402.215204_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.215204
Chromosome:chromosome 17
Location:2699213
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGGTGGCGCCCAGCTGGGGCGCCGTGGG
Internal bar code:CCAGTGTTACTTGATAAGTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:126
LEAP-Seq percent confirming:99.6894
LEAP-Seq n confirming:963
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGGCTATTGAGATGTGC
Suggested primer 2:GTGTGGAACGACATGGTGAG