Insertion junction: LMJ.RY0402.215204_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.215204
Chromosome:chromosome 17
Location:2699311
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCCGCAGGCGGAGCCGGGGTTGGCTGG
Internal bar code:TCCAGACATATCCAGCTTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:876
LEAP-Seq percent confirming:99.7181
LEAP-Seq n confirming:16272
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGGAACGACATGGTGAG
Suggested primer 2:TGCTGGCTATTGAGATGTGC