| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215204 |
| Chromosome: | chromosome_17 |
| Location: | 2699311 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g717950 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTGCCGCAGGCGGAGCCGGGGTTGGCTGG |
| Internal bar code: | TCCAGACATATCCAGCTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 876 |
| LEAP-Seq percent confirming: | 99.7181 |
| LEAP-Seq n confirming: | 16272 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGAACGACATGGTGAG |
| Suggested primer 2: | TGCTGGCTATTGAGATGTGC |