| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215205 |
| Chromosome: | chromosome 5 |
| Location: | 1071162 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246150 | CYG13 | Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAAGCCCTCCGCATCGCGTGCCATCAG |
| Internal bar code: | CGCCTCCGTAAAGAACAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1014 |
| LEAP-Seq percent confirming: | 99.1479 |
| LEAP-Seq n confirming: | 13264 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCAAGGGCTTCACCAAC |
| Suggested primer 2: | GACAGGATTACGTTCCGCAT |