| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215214 |
| Chromosome: | chromosome 16 |
| Location: | 2891157 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663700 | (1 of 1) K03871 - von Hippel-Lindau disease tumor supressor (VHL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCTGCGTGGAGGCAGGGCAGGCGACA |
| Internal bar code: | ACGCAGTAATCGCCCGATTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 99.6183 |
| LEAP-Seq n confirming: | 1044 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCAGGTCGTATCGAGAG |
| Suggested primer 2: | CACCACACGCACATACACAA |