| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215214 |
| Chromosome: | chromosome 16 |
| Location: | 2891219 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663700 | (1 of 1) K03871 - von Hippel-Lindau disease tumor supressor (VHL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGTGCTGAGTCAGCCGGAGCAGCCGCA |
| Internal bar code: | ATAGGGCGAATCCAGGCGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 736 |
| LEAP-Seq percent confirming: | 98.4615 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCAGGTCGTATCGAGAG |
| Suggested primer 2: | CACCACACGCACATACACAA |