| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215241 |
| Chromosome: | chromosome 6 |
| Location: | 3567163 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278118 | 5'UTR|5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGGGACAGGGACCCCCTATGGCGCCAT |
| Internal bar code: | TAGTTGAGAAGGCCATGACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1127 |
| LEAP-Seq percent confirming: | 99.2377 |
| LEAP-Seq n confirming: | 22913 |
| LEAP-Seq n nonconfirming: | 176 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTGTCAACACCGGGAAT |
| Suggested primer 2: | GGTAGCACAACAGGTGAGCA |