| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215241 |
| Chromosome: | chromosome 6 |
| Location: | 3567192 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278118 | 5'UTR|5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGGTAGGGCCTGTACCAAACGACAAGT |
| Internal bar code: | TTTTTGCATTGCAACGGCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1368 |
| LEAP-Seq percent confirming: | 98.431 |
| LEAP-Seq n confirming: | 14617 |
| LEAP-Seq n nonconfirming: | 233 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTGTCAACACCGGGAAT |
| Suggested primer 2: | GGTAGCACAACAGGTGAGCA |