| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215255 |
| Chromosome: | chromosome 15 |
| Location: | 1233314 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641500 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCAGGAGGTGCGTTGGGATGTGCACA |
| Internal bar code: | GGACGTGCCAGCGGCCGGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 98.6524 |
| LEAP-Seq n confirming: | 19473 |
| LEAP-Seq n nonconfirming: | 266 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAAGGTGCTGTCCAAGTG |
| Suggested primer 2: | GTGCGAATAAGGCTCAAAGC |