| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215256 |
| Chromosome: | chromosome 1 |
| Location: | 6687161 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048050 | COA3,COAB1,PPCS1 | (1 of 1) 6.3.2.5 - Phosphopantothenate--cysteine ligase / Phosphopantothenoylcysteine synthetase; Phosphopantothenate-cysteine ligase, CoA biosynthesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGAGCAGCTGCCGGAGGCTGCGGAGCCG |
| Internal bar code: | TTCGCGGTCGTGTGTGTGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 99.655 |
| LEAP-Seq n confirming: | 11266 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGACGCCATTTGAAGAAT |
| Suggested primer 2: | GGGGATGTAGAAATCGCTGA |