| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215257 |
| Chromosome: | chromosome 7 |
| Location: | 2855121 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332100 | (1 of 2) 1.3.3.5 - Bilirubin oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGAATCTGTGCCACGATCACATGTGAG |
| Internal bar code: | GGTTACCCGAAGGTCGTTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.1038 |
| LEAP-Seq n confirming: | 7741 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAGACTTCCGGTAGCAG |
| Suggested primer 2: | ATGAAATCGCTCAGGAATGG |