| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215275 |
| Chromosome: | chromosome 7 |
| Location: | 2359784 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328250 | Pumilio-family RNA binding protein; (1 of 2) K17943 - pumilio RNA-binding family (PUM) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCCTCATCCGCCCTCACAACCTCCACA |
| Internal bar code: | TCCGACCGGTCCAGTTGCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 99.4628 |
| LEAP-Seq n confirming: | 2592 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCTGTGACACGATGGGT |
| Suggested primer 2: | CCCACCCTAAGTTGCAGTGT |