| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215305 |
| Chromosome: | chromosome 14 |
| Location: | 2356888 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623900 | (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTAGCCAGGTCTTGATTGTCTAGTGATG |
| Internal bar code: | TAGGCATTATGCACTGGGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 98.7735 |
| LEAP-Seq n confirming: | 9422 |
| LEAP-Seq n nonconfirming: | 117 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCGTGGACTTCGAATGG |
| Suggested primer 2: | TCCCGGTCCACGACTACTAC |